Dr Yuning Hong | School Seminar


Molecular Reporters for Measuring Proteome Stress in Cells

Maintaining proteostasis is an essential housekeeping function for cell survival. It involves chaperones and degradative pathways to ensure proteins fold correctly and to remove those that are misfolded, damaged or aggregated. In principle, proteostasis is affected in any disease that involves misfolded or mutant proteins that do not fold with normal efficiencies; and hence overdraw on the finite proteostasis resources of the cell. Tracking the proteostasis capacity of cells has the generic potential to track neurodegenerative diseases with diverse specific molecular origins. Hence, building new approaches to identify the efficiency of proteostasis is highly desired in order to track the risk of cells succumbing to damage from protein misfolding and aggregation. In this seminar, I will introduce the new probes and methodology we developed for reporting the changes in protein conformation and modifications resulting from collapse of proteostasis capacity in cells under stress and how we made use of these probes in different models. La Trobe University