Cell-free protein synthesis

We are continuously improving the cell-free expression system available in-house. For example, we can make proteins from linear PCR-amplified DNA in yields as good as those achieved from conventional plasmid DNA. The PCR protocol makes it easy to produce site-directed mutations and we can go from cDNA to protein NMR spectrum in 24 hours. We are currently working to exploit the enzymatic activities of bacterial cell-free extracts to enhance protocols for isotope labelling of proteins using selectively labelled amino acids.