Lanthanide-labelling of proteins

Paramagnetic metal ions, in particular lanthanides, elicit large effects in NMR spectra which provide easily accessible long-range structural information. We are developing generic protocols for site-specific tagging of proteins with a lanthanide-binding peptide or organic molecule. We have developed the capability to incorporate unnatural amino acids site-specifically into proteins and are now working on selective reactions to append lanthanide-binding tags specifically to these amino acids.