The production of ethanol

We are interested in improving the properties of enzyme that for the glycolytic pathway. This is a central metabolic pathway that furnished the cell with energy (in the form of ATP) and numerous useful intermediates. Yeast extends this pathway as part of the process for the production of alcohol. Yeast carefully regulates the production of alcohol - it is only produced in the absence of oxygen and when the level of pyruvate exceeds a critical level. However, it would be useful if the enzymes involved in alcohol production could be deregulated and made to function at low substrate concentrations. This objective has been met with the enzyme pyruvate decarboxylase and in so doing we have also improved he stability of the enzyme.