Thermo Orbitrap Q-Exactive Plus with UHPLC

This instrument couples an analytical flow UHPLC with a Thermo Q-Exactive Plus quadrupole / Orbitrap MS/MS with Electro Spray Ionisation (ESI).

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Dr Adam J. Carroll
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Hardware configuration

LC: Dionex Ultimate 3000 UHPLC (analytical or nano flow)

MS: Thermo Q-Exactive Plus Quadrupole / Orbitrap MS/MS

  • Analytical flow or nanospray ESI
  • Mass resolution up to 280,000 FWHM at m/z 400
  • Mass error <1 ppm
  • Scanning up to 12 spectra / sec
  • MS/MS analysis with CID and HCD fragmentation
  • No intact protein mode
  • Integrated syringe pump for LC-independent sample introduction

Key applications

  • Metabolomics / chemometrics
  • Highly sensitive target quantification
  • Accurate mass characterisation of synthetic molecules
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