Biopolymer and Polymer Facility
The biopolymer and polymer facility is located in the chemical biology section and is a facility for the production of recombinant proteins and characterization of (bio-) polymers.
Content navigation
About
The facility is a “user” facility with a dedicated Technical Officer to manage, maintain and organize renewal of equipment, and train users.
The facility houses comprehensive equipment for small-, medium- and large-scale protein production using in vivo expression and cell-free (in vitro) protein synthesis, as well as instrumentation for sample preparation and storage of biomolecules for structural studies. The facility further complements other analytical facilities in RSC (such as JMSF and MRF) with state-of-the-art instrumentation for (bio-) polymer characterization.
Equipment
Protein production:
- Large scale recombinant protein production: Sartorius Biostat C Plus fermenter
- Medium scale protein production: INFORS HT fermenters and New Brunswick shaker-incubators
- Small scale 96-well high throughput protein production: Heidolf microplate format incubators
- Post-express cell harvesting: large-volume VWR and Beckman centrifuges and bench mounted 96-well plate Eppendorf and Beckman centrifuges
- Cell lysis: Emulsiflex homogenisers (C5 and B15) and sonicator, and French press.
- Protein purification: 7 GE/Cytiva FPLC systems
Protein Characterization:
Various high-value biophysical protein characterization technologies, including:
- Formulatrix microfluidics protein crystallization facility
- Chirascan CD spectrophotometer, Agilent UV-Vi and Fluorescence spectrometers with stopped-flow option
- DAWN Multi Angle Laser Light Scattering
- 3x 96-well plate readers (absorption and fluoresence)
Location
Building 137 Linnaeus Way
Acton, ACT 2601