Research Research equipment Waters Synapt G2-Si HDMS LC-Q/TOF with Ion Mobility Spectrometry Waters Synapt G2-Si HDMS coupled to Waters Acquity UPLC meeting_room Facility Facilities Joint Mass Spectrometry Facility Contact contact_support Contact Contact name Dr Adam J. Carroll Contact email adam.carroll@anu.edu.au Contact number +61 2 6125 7969 About Hardware configuration LC: Waters Acquity Class I Plus UPLC MS: Waters Synapt G2-Si HDMS Quadrupole / Time of Flight MS/MS with Ion Mobility Spectrometry (IMS) Electrospray (ESI) or Atmospheric Pressure Chemical Ionisation (APCI) Mass resolution >50,000 Mass error <1 ppm Scanning up to 30 spectra / sec Capable of detecting large molecules ≥150 kD IMS separates equal m/z ions by size/shape (e.g. conformers) Can perform IMS before or after CID fragmentation Integrated liquid sample infusion system for LC-independent sample introduction Key applications Intact biomacromolecule (e.g. proteins, tRNAs) characterisation – can detect proteins even larger than 150kD Diastereomer and conformer separation with collisional cross section (CCS) determination by IMS Metabolomics / chemometrics Highly sensitive target quantification Characterisation of synthetic chemicals Image