Hardware configuration

LC: Waters Acquity Class I Plus UPLC

MS: Waters Synapt G2-Si HDMS Quadrupole / Time of Flight MS/MS with Ion Mobility Spectrometry (IMS)

• Electrospray (ESI) or Atmospheric Pressure Chemical Ionisation (APCI)
• Mass resolution >50,000
• Mass error <1 ppm
• Scanning up to 30 spectra / sec
• Capable of detecting large molecules ≥150 kD
• IMS separates equal m/z ions by size/shape (e.g. conformers)
• Can perform IMS before or after CID fragmentation
• Integrated liquid sample infusion system for LC-independent sample introduction

Key applications

• Intact biomacromolecule (e.g. proteins, tRNAs) characterisation – can detect proteins even larger than 150kD
• Diastereomer and conformer separation with collisional cross section (CCS) determination by IMS
• Metabolomics / chemometrics
• Highly sensitive target quantification
• Characterisation of synthetic chemicals
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