Diabetes is a chronic metabolic disorder that can cause serious complications without proper monitoring and management strategies. These strategies are currently based upon the use of electrochemical glucose monitors, however, these provide only immediate readings and are not effective at predicting blood sugar fluctuations that are at the root of diabetes complications. Insulin monitoring would provide this much-needed information, however, no insulin sensor is currently commercially available. In this project, we will design a protein-based insulin biosensor to fill this gap. Using computational protein design and molecular biology techniques, we will engineer the binding domain of the human insulin receptor, a transmembrane protein, to express as a soluble unit that can bind insulin outside of the environment of a cell membrane. This construct will then be used as the bioreceptor in an electrochemical sensor. This project will involve collaboration with the ANU Our Health in Our Hands Grand Challenge teams in the College of Engineering & Computer Science and the Research School of Physics.