Designer steroids are synthetically derived compounds that seek to mimic the biological effects of the naturally occurring anabolic androgenic steroid testosterone. In most cases, little is known about the safety, efficacy or metabolic fate of these unusual steroids. A 2015 study of designer steroids identified over 75 products sold legally in some international jurisdictions that contained designer steroids such as those shown below. In the Australian context, the Australian Crime Commission (ACC) figures reveal that the number of steroid arrests and quantity of steroid seized in Australia by the police have increased from 67 and 5.9 kg respectively in 2005-06 to 936 and 17.3 kg in 2013-14 highlighting the growing availability of these agents in the wider community. This project will combine analytical and synthetic chemistry to study the metabolic fate and biological activity arising from designer steroids use, with the goal of developing assays to detect the abuse of these agents in sport.