Continuous Molecular Evolution
This project will make further improvements to targeted gene mutagenesis and apply continuous molecular evolution to enhance specific binding of proteins to their respective targets.
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Protein design makes heavily use of directed molecular evolution, a technique which introduces random mutations and selects for improved variants. The major disadvantage of this method is its high time and labour cost when multiple rounds of selection are performed. With recent development in targeted mutagenesis through CRISPR-CAS9 mediated systems, it becomes possible to perform continuous directed evolution over thousands of generations, thus comprehensively explore and optimise the functional space of proteins. This project will make further improvements to targeted gene mutagenesis and apply continuous molecular evolution to enhance specific binding of proteins to their respective targets.