Proteins are the key targets for pharmaceutical intervention. Most function at relatively low concentration in a crowded cellular environment, which can affect structure and dynamics. This project will magnetically label proteins for new electron paramagnetic resonance (EPR) experiments to study a protein’s structure and dynamics at low concentrations and in-cell, and then compute informative models from the measured EPR distances. These experimental/computational hybrid method offers new and unique capabilities for structural studies of large and/or conformationally flexible systems including those not amenable to established methods, such as Nuclear Magnetic Resonance (NMR), X-ray crystallography and cryogenic electron microscopy (cryo-EM).

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